President Emerson Mnangagwa and opposition MDC Alliance leader Nelson Chamisa have sent their condolence messages following the death of at least 47 people in Rusape on Wednesday evening.
More than 60 people were injured in the accident after two buses collided.
Senior government officials inlcuding Transport Minister Biggie Matiza visited the injured in hospital Thursday morning.
In a statement, President Mnangagwa said the incident was heartbreaking.
"Last night, a heartbreaking tragedy occurred on the Harare-Mutare Road. The great loss of life, the pain and the anguish of the families and victims are today felt by an entire nation," Mnangagwa said.
"At this difficult time, we must come together. Government will ensure the injured receive the treatment they need, and the victims the support they deserve. We will be there for you."
He said government and relevant authorities must investigate what led to the tragic loss of life.
Chamisa, in a statement said the tragic accident was sad and leaves a life-long impact for the families and the country at large.
"Their passing is untimely, the loss of life is sad, painful and leaves life-long negative impact not only for the families but the Zimbabwean community," Chamisa said in a statement issued by MDC Alliance spokesperson Jacob Mafume.
"We share the pain of the families of the deceased. We therefore express our condolences. Our thoughts and prayers are with these families. We recommit the party to contributing to the fight against road carnage."
Meanwhile, government has declared the accident a national disaster.